Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2019-04-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.410, 82.190, 82.660 |
| Unit cell angles | 68.61, 77.36, 77.49 |
Refinement procedure
| Resolution | 46.456 - 1.900 |
| Rwork | 0.171 |
| R-free | 0.21340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fmg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.570 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.460 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.150 | 0.135 |
| Number of reflections | 117709 | 8633 |
| <I/σ(I)> | 9.83 | 1.61 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 6.95 | 6.8 |
| CC(1/2) | 0.997 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium acetate trihydrate, PEG 3,350, 1,5-Diaminopentane dihydrochloride |
