6Y8Y

Structure of Baltic Herring (Clupea Harengus) Phosphoglucomutase 5 (PGM5) with bound Glucose-1-Phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07227
Spacegroup name	P 2 21 21
Unit cell lengths	47.230, 94.680, 146.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.340 - 1.950
R-factor	0.1703
Rwork	0.168
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y8x
RMSD bond length	0.004
RMSD bond angle	0.622
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.116	0.816
Rmeas	0.127	0.898
Rpim	0.051	0.368
Number of reflections	48980	3394
<I/σ(I)>	9.2	2
Completeness [%]	99.9	99.6
Redundancy	6.1	5.7
CC(1/2)	0.996	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.12M calcium acetate, 0.08 M sodium cacodylate pH 6.5, 14.0% (v/v) PEG 8000, 20% (v/v) glycerol