6Y8X

Structure of Atlantic Herring (Clupea Harengus) Phosphoglucomutase 5 (PGM5)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	59.210, 86.220, 63.520
Unit cell angles	90.00, 109.00, 90.00

Refinement procedure

Resolution	55.980 - 2.250
R-factor	0.2394
Rwork	0.237
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5epc
RMSD bond length	0.002
RMSD bond angle	0.473
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MoRDa
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.980	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.232	0.885
Rmeas	0.289	1.102
Rpim	0.171	0.648
Number of reflections	28235	2607
<I/σ(I)>	3.3	1.2
Completeness [%]	98.3	98.5
Redundancy	2.6	2.6
CC(1/2)	0.966	0.221

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1.0 M sodium malonate pH 6.0, 0.1 M MES pH 6.0, 1.0% (v/v) Jeffamine ED-2001