6Y86
Active YidC insertase crystal structure with the first transmembrane domain resolved
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2000-10-30 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 122.590, 122.590, 97.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.994 - 3.400 |
| R-factor | 0.2635 |
| Rwork | 0.262 |
| R-free | 0.30120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | experiment |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS (v1.0) |
| Data scaling software | XDS (v1.0) |
| Phasing software | PHASER (v1.0) |
| Refinement software | PHENIX (dev_2645) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 | 20.000 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 10680 | 10052 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 30.1 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1M Bis-Tris, pH5.5, 0.2M di-ammonium hydrogen citrate with 24% PEG 3350. |
