6Y7F

Crystal structure of human ELOVL fatty acid elongase 7 (ELOVL7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	63.229, 72.459, 112.043
Unit cell angles	90.00, 100.03, 90.00

Refinement procedure

Resolution	28.680 - 2.052
R-factor	0.2112
Rwork	0.210
R-free	0.22610
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	DIALS (2.0.3)
Data scaling software	STARANISO (2.3.25)
Phasing software	AutoSol (1.17.1-3660)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.262	2.182
High resolution limit [Å]	2.052	2.052
Rmerge	0.101	0.703
Rmeas	0.119	0.839
Rpim	0.062	0.449
Number of reflections	36724	1837
<I/σ(I)>	8.3	1.9
Completeness [%]	58.7	17.5
Redundancy	6.9	6.2
CC(1/2)	0.987	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	279	30-36% PEG400 -- 0.1M acetate pH 4.5 -- 0.23M sodium chloride