6Y74
X-ray crystal structure of human carbonic anhydrase IX catalytic domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.930, 78.340, 74.320 |
| Unit cell angles | 90.00, 128.22, 90.00 |
Refinement procedure
| Resolution | 37.130 - 1.530 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.19840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iai |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.130 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.110 | 0.815 |
| Number of reflections | 75610 | 3288 |
| <I/σ(I)> | 10.6 | 2.2 |
| Completeness [%] | 98.3 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 293 | 17% PEG 6000, 1.4 M Sodium citrate pH 4.3, 1 M LiCl |
