6Y6I
lipopolysaccharide outer core galactosyltransferase WaaB and UDP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 81 |
Detector technology | CCD |
Collection date | 2015-02-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 104.440, 104.440, 89.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.850 - 1.920 |
R-factor | 0.1662 |
Rwork | 0.164 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y6g |
RMSD bond length | 0.008 |
RMSD bond angle | 1.375 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.850 | 1.989 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.160 | 1.330 |
Number of reflections | 38400 | 3752 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 23.4 | 11.8 |
CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1% tryptone, 1mM sodium azide, 0.05M HEPES pH7.0 and 20% w/v PEG 3350 |