6Y6G
lipopolysaccharide outer core galactosyltransferase WaaB apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 81 |
Detector technology | CCD |
Collection date | 2015-02-09 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.823 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 104.270, 104.270, 88.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.540 - 1.810 |
R-factor | 0.1646 |
Rwork | 0.163 |
R-free | 0.19560 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.311 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.540 | 1.875 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.125 | 0.863 |
Number of reflections | 44277 | 3879 |
<I/σ(I)> | 29 | |
Completeness [%] | 98.5 | 86.2 |
Redundancy | 60.1 | 18.2 |
CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 1% tryptone, 1mM sodium azide, 0.05M HEPES pH7.0 and 20% w/v PEG 3350. |