6Y6E
drosophila Unr CSD456
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.005 |
| Spacegroup name | P 62 |
| Unit cell lengths | 89.880, 89.880, 58.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.840 - 2.020 |
| Rwork | 0.215 |
| R-free | 0.25130 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.382 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.840 | 2.092 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.087 | 0.860 |
| Rmeas | 0.095 | 0.960 |
| Rpim | 0.037 | 0.410 |
| Number of reflections | 17627 | 1595 |
| <I/σ(I)> | 11.06 | |
| Completeness [%] | 98.7 | 90.07 |
| Redundancy | 6.5 | 5 |
| CC(1/2) | 0.990 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M tri-sodium citrate (pH 5.5), 20% PEG2000 |
