6Y68
Structure of Maporal virus envelope glycoprotein Gc in postfusion conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | H 3 2 |
Unit cell lengths | 90.348, 90.348, 506.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.828 - 2.400 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ljz |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX (1.14rc3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.830 | 39.830 | 2.490 |
High resolution limit [Å] | 2.400 | 8.980 | 2.400 |
Rmerge | 0.174 | 0.052 | 2.752 |
Rmeas | 0.180 | 0.055 | 2.843 |
Rpim | 0.046 | 0.015 | 0.709 |
Total number of observations | 9035 | 52803 | |
Number of reflections | 31958 | 697 | 3310 |
<I/σ(I)> | 11.1 | 35.5 | 1 |
Completeness [%] | 100.0 | 99 | 100 |
Redundancy | 15.1 | 13 | 16 |
CC(1/2) | 0.998 | 0.999 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 30% (w/v) PEG 400, 0.1M CdCl2, 0.1M Na-acetate 4.6 |