Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2017-01-30 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 87.290, 90.410, 100.840 |
Unit cell angles | 94.05, 97.90, 108.06 |
Refinement procedure
Resolution | 45.970 - 1.450 |
Rwork | 0.148 |
R-free | 0.17030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fmg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.513 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.970 | 1.502 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmeas | 0.068 | 0.702 |
Number of reflections | 487522 | 46392 |
<I/σ(I)> | 14.26 | 2.16 |
Completeness [%] | 95.4 | |
Redundancy | 3.6 | |
CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Na nitrate, Na phosphate dibasic, ammonium sulfate, MES, imidazole, PEG 8000, ethylene glycol |