6Y62

Crystal structure of the envelope glycoprotein complex of Maporal virus in a prefusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	120.330, 65.013, 144.757
Unit cell angles	90.00, 99.72, 90.00

Refinement procedure

Resolution	35.144 - 2.200
R-factor	0.1958
Rwork	0.194
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y5f
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.14rc3_3199)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.070	38.070	2.260
High resolution limit [Å]	2.200	9.330	2.200
Rmerge	0.090	0.035	1.254
Rmeas	0.102	0.039	1.526
Rpim	0.047	0.017	0.860
Total number of observations	258945	3312	13723
Number of reflections	55909	743	4505
<I/σ(I)>	11	33.8	0.9
Completeness [%]	99.5	95.2	98.9
Redundancy	4.6	4.5	3
CC(1/2)	0.997	0.998	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M MgCl2, 0.1M TrisHCl 8.5, 17% (w/v) PEG 20K