Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2012-04-30 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.223, 136.416, 85.379 |
| Unit cell angles | 90.00, 109.56, 90.00 |
Refinement procedure
| Resolution | 44.047 - 1.549 |
| Rwork | 0.143 |
| R-free | 0.16920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fmg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.050 | 1.640 |
| High resolution limit [Å] | 1.549 | 1.549 |
| Rmeas | 0.056 | 0.456 |
| Number of reflections | 181696 | 28058 |
| <I/σ(I)> | 16.44 | 2.89 |
| Completeness [%] | 95.3 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Tacsimate, PEG 3350 |
