6Y5F
Crystal structure of the envelope glycoprotein prefusion complex of Andes virus - Mutant H953F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-13 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 188.505, 188.505, 117.278 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.362 | 49.360 | 3.420 |
High resolution limit [Å] | 3.200 | 9.050 | 3.200 |
Rmerge | 0.197 | 0.034 | 2.798 |
Rmeas | 0.204 | 0.035 | 2.888 |
Rpim | 0.050 | 0.009 | 0.709 |
Total number of observations | 13065 | 59067 | |
Number of reflections | 20744 | 1016 | 3657 |
<I/σ(I)> | 13.5 | 60.7 | 1.2 |
Completeness [%] | 99.8 | 98.2 | 99.4 |
Redundancy | 16.2 | 12.9 | 16.2 |
CC(1/2) | 0.999 | 0.999 | 0.532 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 2.4M (NH4)2,HPO4 0.1M TrisHCl pH=8.5 |