6Y4K
Crystal structure of human 14-3-3 gamma in complex with CaMKK2 14-3-3 binding motif Ser100 and Fusicoccin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-24 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 225.852, 225.852, 72.963 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.080 - 3.000 |
R-factor | 0.2616 |
Rwork | 0.260 |
R-free | 0.29210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b05 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.589 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.620 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmeas | 0.165 | |
Number of reflections | 22485 | 3562 |
<I/σ(I)> | 18.91 | 1.01 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 21.83 | 21.01 |
CC(1/2) | 1.000 | 0.495 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG400, HEPES, magnesium chloride, hexafluoropropanol |