6Y4J

Engineered Fructosyl Peptide Oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-05
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	90.033, 90.033, 131.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.770 - 1.380
R-factor	0.1498
Rwork	0.149
R-free	0.16910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t1e
RMSD bond length	0.017
RMSD bond angle	2.082
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	1.420
High resolution limit [Å]	1.350	1.350
Rmerge	0.071	0.407
Rmeas	0.073	0.415
Rpim	0.014	0.083
Number of reflections	118634	17077
<I/σ(I)>	28.8	9.9
Completeness [%]	100.0
Redundancy	20
CC(1/2)	1.000	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M MES monohydrate pH 6.5, 12% PEG 20 K