6Y4B
Structure of cyclodipeptide synthase from Candidatus Glomeribacter gigasporarum bound to Phe-tRNAPhe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 254.834, 254.834, 69.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.160 - 5.000 |
| R-factor | 0.3047 |
| Rwork | 0.304 |
| R-free | 0.31580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mlp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.646 |
| Data reduction software | XDS (1.17.1_3660) |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.300 |
| High resolution limit [Å] | 5.000 | 5.000 |
| Number of reflections | 6120 | 867 |
| <I/σ(I)> | 10.7 | 1 |
| Completeness [%] | 95.6 | 91.6 |
| Redundancy | 33.2 | 32 |
| CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 10% PEG8000, 0.17 M AcCa, 0.17 M Guanidinium hydrochloride, 0.1 M Hepes pH 7 |
