6Y43
Fibrinogen-like globe domain of human ANGPTL6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.382, 65.111, 76.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.530 - 1.600 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qnv |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 76.310 | 76.400 | 1.600 |
| High resolution limit [Å] | 1.570 | 4.260 | 1.570 |
| Rmeas | 0.207 | 0.071 | 1.818 |
| Rpim | 0.062 | 0.021 | 0.676 |
| Total number of observations | 302688 | 18008 | 9396 |
| Number of reflections | 28186 | 1560 | 1360 |
| <I/σ(I)> | 7 | 18.6 | 1.1 |
| Completeness [%] | 100.0 | 100 | 98.3 |
| Redundancy | 10.7 | 11.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Morpheus B5 (0.09M Halogens, 0.1M Buffer System 2 pH 7.5, 50% v/v Precipitant Mix 1) + 25 mM CaCl2 |
