6Y42

Crystal Structure of RsrR complexed to a 39 basepair DNA fragment of the rsrR promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00003
Spacegroup name	P 61 2 2
Unit cell lengths	197.370, 197.370, 73.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.340 - 4.300
R-factor	0.2684
Rwork	0.267
R-free	0.30020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hsd
RMSD bond length	0.010
RMSD bond angle	1.107
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.340	4.450
High resolution limit [Å]	4.300	4.300
Rmerge	0.087	2.130
Rmeas	0.104	2.530
Rpim	0.057	0.988
Number of reflections	6038	3563
<I/σ(I)>	8.8	0.9
Completeness [%]	99.5
Redundancy	5.8
CC(1/2)	0.995	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	NaCl, MgCl2, CaCl2, PEG1000, PEG3350, MES, anaerobic (in glove box)