6Y3G
Crystal structure of phenylalanine tRNA from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 278 |
Detector technology | PIXEL |
Collection date | 2016-09-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 109.618, 109.618, 138.518 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.980 - 3.100 |
R-factor | 0.2163 |
Rwork | 0.215 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yco |
RMSD bond length | 0.004 |
RMSD bond angle | 0.854 |
Data reduction software | XDS (1.17.1_3660) |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.300 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 9432 | 1464 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 12.5 | |
CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 12.4% PEG8000, 0.2M AcCa, 0.1M Hepes pH 7, 0.1M L-Pro |