6Y3D
X-ray structure of thermophilic C-phycocyanin from Galdiera phlegrea
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 60.030, 189.130, 208.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.744 - 1.800 |
| Rwork | 0.221 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3brp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.598 |
| Data reduction software | MOSFLM |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.744 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.199 | 1.800 |
| Rmeas | 0.255 | |
| Rpim | 0.119 | |
| Number of reflections | 217082 | 30323 |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 92.5 | 79.6 |
| Redundancy | 4.4 | 3.6 |
| CC(1/2) | 0.981 | 0.061 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.10 M Hepes, 0.20 M magnesium chloride and 9.0 % (w/v) PEG 4000 |
