6Y3D

X-ray structure of thermophilic C-phycocyanin from Galdiera phlegrea

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.96
Spacegroup name	P 2 21 21
Unit cell lengths	60.030, 189.130, 208.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.744 - 1.800
Rwork	0.221
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3brp
RMSD bond length	0.009
RMSD bond angle	1.598
Data reduction software	MOSFLM
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.744	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.199	1.800
Rmeas	0.255
Rpim	0.119
Number of reflections	217082	30323
<I/σ(I)>	2.7
Completeness [%]	92.5	79.6
Redundancy	4.4	3.6
CC(1/2)	0.981	0.061

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.10 M Hepes, 0.20 M magnesium chloride and 9.0 % (w/v) PEG 4000