6Y31
NG domain of human SRP54 T117 deletion mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.356, 50.607, 181.629 |
| Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
| Resolution | 45.724 - 4.001 |
| R-factor | 0.3124 |
| Rwork | 0.311 |
| R-free | 0.33120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6y2z |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.724 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rpim | 0.213 | 1.250 |
| Number of reflections | 13630 | 1343 |
| <I/σ(I)> | 2.27 | |
| Completeness [%] | 94.6 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.8 M Na2HPO4 , 0.8 M K2HPO4 HEPES 0.1 M (pH 7.5) |
