6Y2Z
NG domain of human SRP54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.443, 87.668, 170.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.371 - 2.150 |
| R-factor | 0.2043 |
| Rwork | 0.202 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l3q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.860 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.371 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rpim | 0.056 | |
| Number of reflections | 42073 | 4140 |
| <I/σ(I)> | 10.75 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | na2hpo4 0.8 M K2HPO4 0.8 M HEPES 0.1 M (pH 7.5) |
