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6Y2T

Streptavidin wildtype with a biotC4-1 cofactor - an artificial iron hydroxylase

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2019-04-12
DetectorDECTRIS EIGER X 16M
Wavelength(s)1.0000
Spacegroup nameI 41 2 2
Unit cell lengths57.714, 57.714, 184.135
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution46.076 - 1.550
Rwork0.169
R-free0.20040
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3pk2
RMSD bond length0.014
RMSD bond angle2.165
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0258)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]46.0761.580
High resolution limit [Å]1.5501.550
Rmerge0.0691.704
Number of reflections232121135
<I/σ(I)>28.8
Completeness [%]100.0100
Redundancy25.3
CC(1/2)1.0000.793
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP42932 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4

217705

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