6Y2S

Escherichia coli R318A RnlA endoribonuclease (single alanine mutant of RnlA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.980126
Spacegroup name	P 21 21 21
Unit cell lengths	64.560, 105.560, 156.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.770 - 3.790
R-factor	0.2677
Rwork	0.264
R-free	0.34180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i8o
RMSD bond length	0.004
RMSD bond angle	0.698
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.770	3.920
High resolution limit [Å]	3.790	3.790
Rmerge		1.250
Number of reflections	10617	941
<I/σ(I)>	3.61	1.1
Completeness [%]	94.4
Redundancy	3.87
CC(1/2)	0.956	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein Buffer: 20 mM Tris HCl pH 8, 150 mM NaCl, 1 mM tris(2-carboxyethyl) phosphine. Reservoir solution: 0.4 M Potassium sodium tartrate tetrahydrate.