6Y2N
Crystal structure of ribonucleotide reductase R2 subunit solved by serial synchrotron crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-02-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.320, 64.320, 153.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.300 - 2.400 |
R-factor | 0.1757 |
Rwork | 0.173 |
R-free | 0.22100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | Unpublished |
RMSD bond length | 0.003 |
RMSD bond angle | 0.492 |
Data reduction software | CrystFEL (0.7.0) |
Data scaling software | CrystFEL (0.7.0) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.300 | 2.580 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 13245 | 2336 |
<I/σ(I)> | 6.73 | |
Completeness [%] | 98.6 | |
Redundancy | 516 | |
CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 4.5 | 294 | 16% (w/v) polyethylene glycol 3350, 2% (v/v) tacsimate pH 4.5 |