6Y2D

Crystal structure of the second KH domain of FUBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9998
Spacegroup name	P 1 21 1
Unit cell lengths	28.888, 82.181, 49.371
Unit cell angles	90.00, 91.04, 90.00

Refinement procedure

Resolution	49.360 - 1.900
R-factor	0.1978
Rwork	0.195
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lij
RMSD bond length	0.016
RMSD bond angle	1.684
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	18030	1152
<I/σ(I)>	5.3
Completeness [%]	99.0
Redundancy	3.9
CC(1/2)	0.990	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2M ammonium sulfate, 5% 2-propanol, 2.5% Glycerol