6Y2C
Crystal structure of the third KH domain of FUBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-28 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 47.409, 47.409, 147.893 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.090 - 2.000 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lij |
RMSD bond length | 0.015 |
RMSD bond angle | 1.455 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.100 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 13595 | 999 |
<I/σ(I)> | 8.3 | |
Completeness [%] | 98.8 | |
Redundancy | 4.5 | |
CC(1/2) | 0.995 | 0.661 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 6000, 10% ethylene glycol, 0.015M Zinkchlorid, 0.1 M MES, pH 6.0 |