6Y22
RING-DTC domains of Deltex 2, Form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97997 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 106.418, 106.418, 74.669 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.008 - 2.069 |
R-factor | 0.1807 |
Rwork | 0.179 |
R-free | 0.20830 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.836 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.10_2142) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.160 | 2.120 |
High resolution limit [Å] | 2.069 | 2.069 |
Rmerge | 0.076 | 0.748 |
Rpim | 0.037 | 0.360 |
Number of reflections | 29929 | 2130 |
<I/σ(I)> | 16.5 | 3.3 |
Completeness [%] | 99.7 | 97.3 |
Redundancy | 10 | 10 |
CC(1/2) | 0.999 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 292 | Morpheus condition 60: 0.12 M ethylene glycols (0.3M Diethylene glycol; 0.3M Triethylene glycol; 0.3M Tetraethylene glycol; 0.3M Pentaethylene glycol), 0.1 M Buffer system 3 (1 M Tris-Bicine pH 8.5), 37.5% Precipitation mix 4 (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350) |