6Y1V

Mycobacterium tuberculosis FtsZ-GTP-gamma-S in complex with 4-hydroxycoumarin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	88.539, 88.539, 180.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.680 - 2.400
R-factor	0.1752
Rwork	0.172
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rq7
RMSD bond length	0.013
RMSD bond angle	1.399
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	180.300	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	31240	4542
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	4.3
CC(1/2)	0.992	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291.15	27.5% PEG4000, 0.4 M ammonium acetate and 0.1 M Na citrate soaked with 37.5 mM of inhibitor