6Y1G
Photoconverted HcRed in its optoacoustic state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999995 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.710, 75.200, 254.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.780 - 2.300 |
| R-factor | 0.1717 |
| Rwork | 0.168 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yzw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.965 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 44173 | 3184 |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 13.3 | 13.3 |
| CC(1/2) | 0.995 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1 M BICINE pH 8.5, 22% w/v Polyethylene glycol 10,000 |
