6Y1D

Binary complex of 14-3-3 sigma (C38N) with the Estrogen Related Receptor gamma (DBD) phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	82.261, 111.949, 62.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.618 - 1.380
R-factor	0.1659
Rwork	0.165
R-free	0.18730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p1n
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BUSTER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.290	1.400
High resolution limit [Å]	1.380	1.380
Number of reflections	59105	2440
<I/σ(I)>	26.2	2.6
Completeness [%]	98.8
Redundancy	12.3
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.095 M HEPES (pH 7.3), 0.19 M CaCl2, 25% (v/v) PEG 400 and 5% (v/v) glycerol