6Y12

Arginine hydroxylase VioC in complex with (3S)-OH-Arg, succinate and Fe after oxygen exposure using FT-SSX methods

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-09-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96863
Spacegroup name	C 1 2 1
Unit cell lengths	82.369, 67.125, 63.984
Unit cell angles	90.00, 110.15, 90.00

Refinement procedure

Resolution	60.070 - 1.700
R-factor	0.184
Rwork	0.182
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wbo
RMSD bond length	0.004
RMSD bond angle	0.698
Data reduction software	DIALS (1.8)
Data scaling software	DIALS (1.8)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.080	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	36065	3574
<I/σ(I)>	34.19	2.34
Completeness [%]	99.9	99.39
Redundancy	62.41
CC(1/2)	0.949	0.255

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	298	0.05M MgCl2, 26% PEG550, 0.1M HEPES