6Y11
Respiratory complex I from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.280, 340.890, 263.300 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 58.666 - 3.109 |
| R-factor | 0.211 |
| Rwork | 0.204 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hea |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.881 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.670 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 300514 | 14755 |
| <I/σ(I)> | 6.7 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.2 | 15 |
| CC(1/2) | 0.998 | 0.306 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM |
