6Y0Y
Sarcin Ricin Loop, mutant C2666U
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 22.480, 27.550, 29.690 |
| Unit cell angles | 91.58, 93.61, 108.31 |
Refinement procedure
| Resolution | 21.281 - 0.950 |
| R-factor | 0.1259 |
| Rwork | 0.125 |
| R-free | 0.13870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dvz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.266 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 0.920 |
| High resolution limit [Å] | 0.890 | 0.890 |
| Number of reflections | 47148 | 2093 |
| <I/σ(I)> | 13.27 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.998 | 0.263 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 5mM MgCl2, 50mM Na HEPES pH 7.0, 25% PEG-MME 550 |
