6Y0T

Sarcin Ricin Loop, mutant C2666A U2653G C2667A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	53.170, 23.960, 63.820
Unit cell angles	90.00, 108.74, 90.00

Refinement procedure

Resolution	33.720 - 1.393
R-factor	0.1675
Rwork	0.166
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.006
RMSD bond angle	1.215
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.720	1.430
High resolution limit [Å]	1.390	1.390
Number of reflections	30750	1911
<I/σ(I)>	15.4
Completeness [%]	98.8
Redundancy	13.1
CC(1/2)	0.999	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	80mM KCl, 20mM MgCl2, 40mM Na cacodylate pH 6.0, 46% MPD, 12mM spermine tetrahydrochloride