6Y0N
Arginine hydroxylase VioC in complex with Arg, 2OG and Fe under anaerobic environment using FT-SSX methods
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.990, 66.578, 63.584 |
| Unit cell angles | 90.00, 110.15, 90.00 |
Refinement procedure
| Resolution | 59.690 - 1.860 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wbo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.612 |
| Data reduction software | DIALS (1.8) |
| Data scaling software | DIALS (1.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.692 | 1.892 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 27964 | 1398 |
| <I/σ(I)> | 28.79 | 2.25 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 86.89 | |
| CC(1/2) | 0.944 | 0.279 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 0.05M MgCl2, 26% PEG 550, 0.1M HEPES |
