6Y0L

Crystal structure of human CD23 lectin domain N225D, K229E, S252N, T251N, R253G, S254G mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97945
Spacegroup name	P 6 2 2
Unit cell lengths	113.704, 113.704, 45.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.852 - 1.650
R-factor	0.1686
Rwork	0.167
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g9a
RMSD bond length	0.012
RMSD bond angle	1.246
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.480	1.730
High resolution limit [Å]	1.650	1.650
Rmerge	0.094
Rpim	0.028	0.413
Number of reflections	20443
<I/σ(I)>	16.6	1.6
Completeness [%]	94.4	99.8
Redundancy	11.5	5.6
CC(1/2)	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.0M ammonium sulphate, 0.2M sodium chloride, 0.1M sodium-cacodylate, 8mM CaCl2