6Y0H
High resolution structure of GH11 xylanase from Nectria haematococca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.446, 38.500, 53.591 |
| Unit cell angles | 90.00, 91.43, 90.00 |
Refinement procedure
| Resolution | 34.670 - 1.000 |
| R-factor | 0.1423 |
| Rwork | 0.142 |
| R-free | 0.15350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jrm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.393 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.670 | 1.060 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Number of reflections | 82307 | 11904 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 93.3 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 1 M Ammonium sulphate,100mM Sodium citrate |
