6Y01
The structure of the molybdenum cofactor binding protein from the phototrophic bacterium Rippkaea orientalis
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-17 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.577, 72.044, 70.264 |
| Unit cell angles | 90.00, 113.33, 90.00 |
Refinement procedure
| Resolution | 64.518 - 1.230 |
| Rwork | 0.142 |
| R-free | 0.16290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iz6 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.845 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.520 | 1.274 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.053 | 3.026 |
| Rmeas | 0.057 | 3.299 |
| Rpim | 0.022 | 1.297 |
| Number of reflections | 181674 | 18104 |
| <I/σ(I)> | 16.35 | 0.55 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.3 |
| CC(1/2) | 1.000 | 0.193 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 15% (v/v) Pentaerythritol ethoxylate; 0.2 M potassium acetate; 3% (v/v) Jeffamine T-403; 0.1 M MES pH 6.0 |
