6XZN
Arabidopsis UV-B photoreceptor UVR8 mutant G101S W285A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-12 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 50.541, 99.123, 132.727 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.400 - 1.750 |
R-factor | 0.1585 |
Rwork | 0.155 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d9s |
RMSD bond length | 0.010 |
RMSD bond angle | 1.057 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.400 | 1.813 |
High resolution limit [Å] | 1.750 | 1.751 |
Number of reflections | 67187 | 6556 |
<I/σ(I)> | 21.71 | |
Completeness [%] | 98.7 | |
Redundancy | 13.4 | |
CC(1/2) | 1.000 | 0.659 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M Sodium nitrate 0.1M Bis Tris propane pH 8.5 20% w/v PEG 3350. 20% Glycerol cryoprotectant. |