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6XZL

Arabidopsis UV-B photoreceptor UVR8 mutant D96N D107N

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyPIXEL
Collection date2016-12-19
DetectorDECTRIS PILATUS 2M-F
Wavelength(s)1
Spacegroup nameC 1 2 1
Unit cell lengths97.614, 50.980, 70.488
Unit cell angles90.00, 104.95, 90.00
Refinement procedure
Resolution47.160 - 1.390
R-factor0.1458
Rwork0.144
R-free0.17930
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4d9s
RMSD bond length0.010
RMSD bond angle1.028
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwarePHENIX (1.17.1_3660)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]47.1601.440
High resolution limit [Å]1.3901.390
Number of reflections674246711
<I/σ(I)>8.97
Completeness [%]100.0
Redundancy6.6
CC(1/2)0.9960.599
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP293.15Methylated protein. 0.1M TRIS 8.5, 0.1M NaCl, 30% PEG4000, 1.8mg/mL, 2:1, prot:buffer. 25% glycerol cryoprotectant

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