6XZL
Arabidopsis UV-B photoreceptor UVR8 mutant D96N D107N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-19 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.614, 50.980, 70.488 |
Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
Resolution | 47.160 - 1.390 |
R-factor | 0.1458 |
Rwork | 0.144 |
R-free | 0.17930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d9s |
RMSD bond length | 0.010 |
RMSD bond angle | 1.028 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.160 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Number of reflections | 67424 | 6711 |
<I/σ(I)> | 8.97 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 | |
CC(1/2) | 0.996 | 0.599 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Methylated protein. 0.1M TRIS 8.5, 0.1M NaCl, 30% PEG4000, 1.8mg/mL, 2:1, prot:buffer. 25% glycerol cryoprotectant |