6XYB
Crystal structure of Q4D6Q6, a conserved kinetoplastid-specific protein from Trypanosoma cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.701, 44.487, 69.544 |
| Unit cell angles | 90.00, 93.46, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.470 |
| R-factor | 0.1454 |
| Rwork | 0.143 |
| R-free | 0.18550 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.954 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.710 | 1.550 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmeas | 0.067 | 0.824 |
| Number of reflections | 70953 | 11262 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.4 | 98.2 |
| Redundancy | 3.7 | 3.5 |
| CC(1/2) | 0.998 | 0.642 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 5000 MME, 0.2 M potassium iodide |
