6XYA

Cap-binding domain of SFTSV L protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	38.974, 44.715, 63.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.220 - 1.350
R-factor	0.166
Rwork	0.165
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qhg
RMSD bond length	0.009
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.398
High resolution limit [Å]	1.350	1.350
Rmerge	0.031	0.368
Rmeas	0.032	0.386
Number of reflections	321482	25283
<I/σ(I)>	41.6	6.32
Completeness [%]	97.6	88.8
Redundancy	13.2	11.6
CC(1/2)	1.000	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Final concentrations of components: 16.6 mM Sodium phosphate (pH 6.5), 50mM NaCl, 6.6% (w/v) Glycerol, 4.7% (v/v) 2-Butanol, 100mM Mes (pH 6.0), 18.3% (w/v) Polyethylene glycol 4000, 1.3 mM m7GTP Protein concentration: SFTSV CBD 12.4 mg/ml