6XX9

Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	53.410, 62.220, 62.080
Unit cell angles	90.00, 106.75, 90.00

Refinement procedure

Resolution	42.982 - 1.840
R-factor	0.200485110195
Rwork	0.199
R-free	0.22681
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pzh
RMSD bond length	0.005
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.837	1.840
Number of reflections	33228	4699
<I/σ(I)>	12
Completeness [%]	97.4
Redundancy	4.4
CC(1/2)	0.999	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium sulfate 0.1M Bis-Tris propane pH 6.5 20% PEG 3350