6XX7

Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal in complex with ANP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	60.560, 60.560, 228.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.410 - 2.400
R-factor	0.1979
Rwork	0.195
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pzh
RMSD bond length	0.003
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.14_3260)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.400	2.400
Number of reflections	17625	2711
<I/σ(I)>	10.2
Completeness [%]	99.6
Redundancy	16.6
CC(1/2)	0.997	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium sulfate 0.1M Bis-Tris propane pH 6.5 20% PEG 3350