6XWT

drosophila melanogaster CENP-A/H4 bound to N-terminal CAL1 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97626
Spacegroup name	H 3 2
Unit cell lengths	178.250, 178.250, 133.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.880 - 3.470
R-factor	0.2724
Rwork	0.272
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pyo
RMSD bond length	0.004
RMSD bond angle	0.865
Data reduction software	xia2 (1.17.1_3660)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.880	3.590
High resolution limit [Å]	3.470	3.470
Rmerge	0.147	1.960
Number of reflections	10675	1059
<I/σ(I)>	10.46
Completeness [%]	99.9
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.01M Cobalt (II) Chloride hexahydrate, 0.1M MES pH 6.5 and 1.8 M Ammonium Sulphate