6XWF

Crystal structure of an NCoR1BBD2-BCL6BTB chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.96863
Spacegroup name	P 61 2 2
Unit cell lengths	68.940, 68.940, 166.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.220 - 1.600
R-factor	0.1915
Rwork	0.190
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r28
RMSD bond length	0.005
RMSD bond angle	0.709
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.700	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.194
Rpim	0.033	0.511
Number of reflections	31859	4539
<I/σ(I)>	14.3
Completeness [%]	100.0	100
Redundancy	33.6	34.6
CC(1/2)	0.999	0.465

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	2.2M NaCl, 10% MPD, 0.1M imidazole pH 6.5, 0.1M CaCl2