6XUW
Human myelin protein P2 mutant L27D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.880, 64.880, 100.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.310 |
| R-factor | 0.1908 |
| Rwork | 0.188 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wut |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.530 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2140) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.370 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmeas | 0.232 | 2.208 |
| Number of reflections | 9951 | 653 |
| <I/σ(I)> | 8 | 0.9 |
| Completeness [%] | 98.9 | 94.6 |
| Redundancy | 7.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 38% PEG 6000, 0.1 M citrate, pH 5.0 |
