6XUV

Crystallographic structure of oligosaccharide dehydrogenase from Pycnoporus cinnabarinus, laminaribiose-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9685
Spacegroup name	P 21 21 21
Unit cell lengths	48.914, 60.929, 194.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.330 - 1.750
R-factor	0.16977
Rwork	0.168
R-free	0.20729
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.014
RMSD bond angle	2.005
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.330	2.020
High resolution limit [Å]	1.750	1.750
Number of reflections	59350	20470
<I/σ(I)>	7
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	2 M ammonium sulfate